Helical indexing (layer-lines)


You may find this part difficult to learn. That's fine, everyone feels the same at the beginning.
Plus, there may be five people fully understanding this in the world. You could be the sixth.

Finish those readings before you look at FAQs.

1988-Stewart

Computer Image Processing of Electron Micrographs of Biological Structures with Helical Symmetry

A good start to understand power spectrum

PDF

2005-DeRosier

A tutorial on helical structures

Some examples given by David

PDF

2014-Egelman

Ambiguities in helical reconstruction

Understand power spectrum in 3D

PDF

2020-Egelman&Wang

cryo-EM of helical polymers

Some concepts and common pitfalls

PDF

Let's walk through those questions

What is a power spectrum?

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How should the power spectrum be generated  (for estimating possible helical symmetries)

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Can you determine the helical symmetry from the power spectrum

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The KEY equation? 

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What we do

Use cryo-EM to understand and design large assemblies

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