Helical indexing (layer-lines)

You may find this part difficult to learn. That's fine, everyone feels the same at the beginning.
Plus, there may be five people fully understanding this in the world. You could be the sixth.

Finish those readings before you look at FAQs.


Computer Image Processing of Electron Micrographs of Biological Structures with Helical Symmetry

A good start to understand power spectrum



A tutorial on helical structures

Some examples given by David



Ambiguities in helical reconstruction

Understand power spectrum in 3D



cryo-EM of helical polymers

Some concepts and common pitfalls


Let's walk through those questions

What is a power spectrum?

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How should the power spectrum be generated  (for estimating possible helical symmetries)

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Can you determine the helical symmetry from the power spectrum

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The KEY equation? 

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What we do

Use cryo-EM to understand and design large assemblies

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